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Spécifications
- Antibody type:Primary
- Antigen name:Tubulin Beta 3
- Clonality:Monoclonal
- Clone:TU-20
- Gene ID:10381
- Host:Mouse
- Isotype:IgG1
- Reactivity:Baboon,Hamster,Carp,Pig,Mammals,Human,Bovine,Rat,Mouse
- Antigen symbol:TUBB3
- Conjugation:Unconjugated
- Flow cytometry:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:Tubulin beta-3 chain|CDCBM|beta-4|CFEOM3A|FEOM3|CDCBM1|TUBB4|CFEOM3
- Storage buffer:Phosphate buffered saline pH 7.4 containing 15mM sodium azide
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Immunogen:Synthetic peptide: ESESQGPK conjugated to malemide activated KLH, corresponding to amino acids 441-448 of Human Tubulin.
- Tested applications:Fluorescence-Activated Cell Sorting
- Purification:>95% by SDS-PAGE
- Cat. no.:89362-648
- Supplier No.:GTX27751
Spécifications
A propos de cet article
Mouse monoclonal antibody [TU-20] to beta III Tubulin
Flow Cytometry: Use at an assay dependant concentration
Western Blotting
Recommended dilution:1-2 µg/ml, 90 min
Positive control: Porcine brain lysate
Negative control: HPB-ALL human peripheral blood leukemia cell line
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry (paraffin sections)
Recommended dilution: 10 µg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or Hightemperature citrate buffer antigen retrieval Positive tissue: neuronal tissue
Immunocytochemistry
Positive material: Neuro2a mouse neuroblastoma cell line
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: TUBB3
Clonality: Monoclonal
Clone: TU-20
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Mouse, Pig, Rat, Bb, Carp, Hamster, Mammals, Bovine