To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Spécifications
- Antibody type:Primary
- Antigen name:NFAT1
- Clonality:Monoclonal
- Clone:25A10.D6.D2
- Gel shift:Yes
- Gene ID:18019
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:Nfatc2
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:100 μg
- Western blot:Yes
- Format:Ig fraction
- Amino acid number:51 to 69
- Storage buffer:Phosphate-buffered saline containing 0.05% sodium azide
- Sequence:AISSPSGLAYPDDVLDYGL
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Synthetic peptide: (51)AISSPSGLAYPDDVLDYGL(69). The immunizing peptide corresponds to amino acid residues 51-69 of mouse NFAT1-A, B and C isoforms. The sequence of this N-terminal peptide is ~70% homologous with human NFAT1.
- Tested applications:Gel Supershift Assay
- Purification:IgG fraction
- Cat. no.:89367-548
- Supplier No.:GTX22722
Spécifications
A propos de cet article
The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
Recommended Starting Dilutions:ICC/IF: 1:100 - 1:1000ChIP assay: 1-3 ulIHC-P|IHC-Fr: 1:50 - 1:200IHC: 1 ug/mlWB: 1:1000GSA: 67 ug/mlNot tested in other applications. Optimal dilution / concentration should be determined
Type: Primary
Antigen: Nfatc2
Clonality: Monoclonal
Clone: 25A10.D6.D2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Mouse, Rat