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Spécifications
- Antibody type:Primary
- Antigen name:Phosphodiesterase 1C, Calmodulin-Dependent 70kDa
- Clonality:Polyclonal
- Gene ID:5137
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human
- Antigen symbol:PDE1C
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:Hcam3|cam-PDE 1C|hCam-3
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:0.75 mg/ml
- Immunogen:Synthetic peptide (N terminal).
- Tested applications:ICC/IF, IP, WB
- Purification:Immunogen affinity purified
- Cat. no.:89367-332
- Supplier No.:GTX14602
Spécifications
A propos de cet article
Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodiesterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1C gene has been cloned from human, mouse and rat. The PDE1C gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. The tissue distribution of PDE1C is different from other PDE1 gene family members. A 5 splice variant of PDE1C2 is found in rat olfactory epithelium and is believed to be involved in olfactory signal transduction. Other PDE1C variants (PDE1C1 and PDE1C2) are found in human heart. The PDE1C enzymes bind both cGMP and cAMP with different affinities.
For ICC: Use at a dilution of 1:200. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/250 - 1/500. This antibody labels multiple bands in the 70-87 kDa range all believed to be various PDE1C variants. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: PDE1C
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human