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Spécifications
- Antibody type:Primary
- Antigen name:Actin Alpha 1, Skeletal Muscle
- Clonality:Monoclonal
- Clone:2Q1055
- Gene ID:396526
- Host:Mouse
- Isotype:IgG1
- Reactivity:Chicken,Human
- Antigen symbol:ACTA1
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:50 μg
- Western blot:Yes
- Antigen synonyms:ACT1|alpha skeletal muscle Actin|ASMA|alpha Actin 1|NEM2|ACTA|NEM1
- Storage buffer:Preservative: 0.1% Sodium Azide; Constituents: 40% Glycerol, 10mM PBS. pH 7.6
- Storage temperature:Upon receipt - Keep as concentrated solution. Aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:0.5 mg/ml
- Immunogen:Chicken gizzard actin
- Tested applications:ELISA, ICC/IF, IHC-P, WB
- Purification:Protein A purified
- Cat. no.:89359-952
- Supplier No.:GTX14128
Spécifications
A propos de cet article
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
ELISA: Use at an assay dependent dilution. In ELISA, shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoidum and Physarum polycephalum in ELISA. ICC: Use at a concentration of 10 ?g/ml on methanol fixed mouse 3T3 cells. IHC-P: Use at a concentration of 10 ?g/ml. WB: Use at a concentration of 1 - 20 ?g/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Predicted molecular weight: 43 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: ACT1
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: