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Spécifications
- Antibody type:Primary
- Antigen name:Thymepoietin
- Clonality:Monoclonal
- Clone:6E10
- Gene ID:7112
- Host:Mouse
- Isotype:IgG1
- Reactivity:Bovine
- Antigen symbol:LAP2
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- Size:100 μl
- Western blot:Yes
- Antigen synonyms:LEMD4|TP|áCMD1T|Lamina-associated polypeptide 2|isoforms beta/gamma|PRO0868|LAP2
- Amino acid number:29 to 50
- Storage buffer:Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4
- Sequence:GEQRKDVYVQLYLQHLTARNRP
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:2.3 mg/ml
- Immunogen:Synthetic peptide: GEQRKDVYVQLYLQHLTARNRP, corresponding to amino acids 29-50 of Human LAP2, common to all isoforms.
- Tested applications:IHC-P
- Purification:Protein A purified
- Cat. no.:89358-860
- Supplier No.:GTX11823
Spécifications
A propos de cet article
Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP1), and several isoforms of LAP2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP2 beta and the nucleoplasmic protein LAP2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth , LAP2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.
IHC-P: Use at an assay dependent dilution. WB: Use at a concentration of 2 - 4 ?g/ml. Detects bands of approximately 76 (alpha), 50 (beta, singlet or doublet), 45 (epsilon), 42 (delta), 38 (gamma, singlet or doublet) and 25 kDa (zeta). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: LAP2
Clonality: Monoclonal
Clone: 6E10
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Bovine