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Spécifications
- Antibody type:Primary
- Antigen name:Hepatocyte growth factor
- Clonality:Monoclonal
- Clone:24612.111
- Gene ID:3082
- Host:Mouse
- Isotype:IgG1
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:HGF
- Conjugation:Unconjugated
- ELISA:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:Hepatocyte growth factor|HPTA|DFNB39|HGFB|Hepatopoeitin-A|Fibroblast-derived tumor cytotoxic factor|Lung fibroblast-derived mitogen|SF|F-TCF
- Storage buffer:PBS containing 5% Trehalose
- Storage temperature:Aliquot and store at –20 °C or –80 °C. Avoid repeated freeze / thaw cycles.
- Concentration:0.5mg/ml
- Immunogen:Recombinant human hepatocyte growth factor (rhHGF) expressed in the insect cell line Sf 21.
- Tested applications:ELISA, Neut, WB
- Purification:Protein A purified
- Cat. no.:89354-780
- Supplier No.:GTX10678
Spécifications
A propos de cet article
Mouse monoclonal [24612.111] to HGF
ELISA: Use at a concentration of 0.5 - 1 ?g/ml to detect <1.2 ng/well of rhHGF. Neut: Use at a concentration of 0.01 - 0.5 ?g/ml. WB: Use at a concentration of 1 - 2 ?g/ml to detect rhHGF at 50 ng/lane under non-reducing conditions. Predicted molecular weight: 83.1 kDa. Monoclonal Anti-Human HGF was tested for its ability to neutralize the bioactivity of rhHGF in a cell proliferation assay using 4MBr-5 cells, a monkey epithelial cell line responsive to HGF. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhHGF that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 100 ng/ml rhHGF was preincubated with various dilutions of the antibody for 1 hour at 22°C, then placed in a 96-well microtiter plate. 4MBr-5 cells were added to each well and incubated for 48 hours at 37°C in a 5% CO2 humidified incubator and then pulsed for the last 24 hours with ^3H-thymidine. Cells were harvested onto glass filters and the ^3H-thymidine incorporation into DNA was measured. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: HGF
Clonality: Monoclonal
Clone: 24612.111
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human