A propos de cet article
Biotium's apoptosis/necrosis quantitation kits pair green fluorescent FITC-Annexin V conjugate with the membrane impermeant red fluorescent nucleic acid stain ethidium homodimer III
Green fluorescent CF™488A is brighter and more photostable than FITC, and unlike FITC, CF™488 fluorescence is not pH sensitive. Biotium offers CF™488A Annexin V conjugate in combination with propidium iodide or 7-AAD, membrane impermeant nucleic acid vital dyes that stain dead cell with red fluorescence. Propidium iodide is recommended for flow cytometry, while 7-AAD is recommended for fluorescence microscopy due to minimal fluorescence spill-over of 7-AAD in the green channel.
Features:
Fluorescently stain apoptotic cells green and necrotic cells red.
CF™488A Annexin V and other Annexin V conjugates of Biotium's bright and photostable CF™ Dyes also are available as stand-alone products. See catalog number 89138-858.
In the Apoptosis and Necrosis Quantitation Kit, FITC-Annexin V stains phosphatidylserine on the surface of apoptotic cells, while ethidium homodimer III stains necrotic and late apoptotic cells that have compromised plasma membrane integrity. Ethidium homodimer III also allows visualization of dead cell nuclear morphology to distinguish late apoptotic cells from necrotic cells. The Apoptotic, Necrotic and Healthy cells Quantitation Kit includes FITC-Annexin V, ethidium homodimer III, and Hoechst 33342 DNA dye, which stain the nuclei of all cells with blue fluorescence. The kits can be used for detection by microplate reader, flow cytometry, or fluorescence microscopy.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.