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Spécifications
- Size:500 g
- Cat. no.:97063-454
- Application:Recombinant E. coli Strains Preparation
- Supplier No.:J906-500G
Spécifications
A propos de cet article
SOB broth powder is used for the preparation of nutritionally rich growth medium and transformation of competent E
SOB Broth (Super Optimal Broth) growth medium is nutritionally rich, containing casein hydrolysate and yeast extract as sources of nitrogenous compounds and growth factors,and magnesium sulfate as a supply of magnesium ions necessary for DNA replication.
when dissolved at 25.5 g per liter of water,yields the formulation
Tryptone -------- 20 Grams/L
Yeast Extract- - -5 Grams/L
Sodium Chloride--0.5 Gram/L
Medium should be autoclaved or sterile-filtered. Allow the solution to cool to 50 °C or below before adding supplemental components and antibiotic. Add 20 mL of 1 M MgSO4.Store SOB Broth powder at room temperature (18 – 26 °C). Store prepared medium cold (2 – 8 °C).
coli.
Fournisseur: VWR International
184,04 $ to 11 818,61 $
Fournisseur: VWR International
194,14 $ to 694,12 $