A propos de cet article
EconoTaq® DNA Polymerase is an excellent Taq polymerase for routine PCR
EconoTaq® DNA Polymerase is a high performance Taq polymerase offered with your choice of reaction buffers, with or without MgCl2. EconoTaq® DNA Polymerase also offers exceedingly high lot-to-lot reproducibility and reliability.
Concentration: 5 units/µl. One unit catalyzes the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 70°C in 50mM Tris-HCl (pH 9.0), 50mM NaCl, 5mM MgCl2, 200µM dGTP, dATP, dTTP dCTP (a mix of unlabeled and [33P] dCTP), 10µg of activated calf thymus DNA, and 0.1mg/ml BSA. Storage Buffer: 10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1% Triton X-100, 0.1mM EDTA, 1mM DTT, and 50% glycerol. 10X Reaction Buffer: 100mM Tris-HCl (pH 9.0), 500mM KCl, 1% Triton X-100, and with or without 15mM MgCl2. PCR Activity: EconoTaq® DNA Polymerase is tested in DNA amplification using a variety of templates and primers.
Activity Determination: One unit of EconoTaq® DNA Polymerase catalyzes the incorporation of 10nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50mM Tris-HCl (pH 9.0), 50mM NaCl, 5mM MgCl2, 200µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10µg Activated Calf Thymus DNA, and 0.1mg/ml BSA. Absence of Endoonuclease or Nicking Activity: Incubation of 10U of EconoTaq® DNA Polymerase with 1µg of supercoiled pBR322 DNA for 16 hours at 70°C results in no detectable conversion to relaxed or linear forms detectable by agarose gel electrophoresis. Absence of Exonuclease Activity: Incubation of 10U of EconoTaq® DNA Polymerase with 1µg of HindIII-cut lambda DNA for 16 hours at 70°C resulted in no smearing of bands on agarose gels. Purity: EconoTaq® DNA Polymerase is >99% pure as determined by SDS PAGE. There is no detectable DNA contamination.
It performs just as well as more expensive alternatives, including Tfl
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.