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Spécifications
- Description:QuantiFluo™ α-Amylase inhibitor screening kit
- Size:400 tests
- Cat. no.:77441-692
Spécifications
A propos de cet article
For evaluation of drugs and screening potential inhibitors of α-amylase.
- Safe
- Non-radioactive assay
- Fast and convenient
- Homogeneous 'mix-incubate-measure' type assay
- Can be completed in under an hour at room temperature
- Robust and High-throughput
- A Z’-factor of >0.90 was observed in a 384-well format
- Can be readily automated to assay thousands of samples per day
AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose, and 'limit dextrin' from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis, and renal failure.
Simple, direct, and automation-ready procedures for measuring α-amylase inhibition are highly desirable in Research and Drug Discovery. BioAssay Systems’ QuantiFluo™ α-Amylase Inhibitor Screening Kit utilizes fluorescence polarization (FP) to screen for potential α-amylase inhibitors. In this assay, α-amylase cleaves a fluorescent amylose substrate. The decrease in FP is directly proportional to the α-amylase activity in the sample. Inhibition is therefore determined by the increase in FP (λex/em = 485/520 nm).