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Spécifications
- Description:QuantiChrom™ Pyruvate dehydrogenase assay kit
- Size:100 tests
- Cat. no.:77441-660
Spécifications
A propos de cet article
For assessing PDH activity in biological samples and screening and evaluation of PDH modulators.
- High sensitivity and wide linear range
- The detection limit is 0.87 U/L, with linearity up to at least 278 U/L PDH in a 96-well plate assay
- Homogeneous and simple procedure
- Simple 'mix-and-measure' procedure allows reliable quantitation of PDH activity within 20 minutes
- Robust and amenable to HTS
- All reagents are compatible with high-throughput liquid handling instruments
PYRUVATE DEHYDROGENASE (PDH) (E.C. 1.2.4.1) is an oxidoreductase which catalyzes the decarboxylation of pyruvate to form acetylated dihydrolipoamide in a thiamine pyrophosphate (TPP)-dependent reaction. PDH is the E1 component of the PDH complex that ultimately produces acetyl-CoA. The PDH complex connects glycolysis to the citric acid cycle. This non-radioactive colorimetric PDH assay is based on the reduction of the tetrazolium salt MTT in a Phenazine Methosulfate (PMS) coupled reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the PDH activity.