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Spécifications
- Description:QuantiFluo™ trypsin inhibitor assay kit
- Size:100 tests
- Cat. no.:77441-670
Spécifications
A propos de cet article
For evaluation of drugs and screening of potential inhibitors to trypsin proteases.
- Safe and non-radioactive assay
- Homogeneous and convenient
- Homogenous 'mix-incubate-measure' type assay
- No wash and reagent transfer steps are involved
- Robust and high-throughput
- Can be readily automated to assay thousands of samples per day
- Robust assay with a Z factor of >0.7
Trypsin (EC 3.4.21.4) is a digestive, serine protease that hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. Trypsin predominantly cleaves peptide chains at the carboxyl side of lysine and arginine amino acids, but not before proline. BioAssay systems QuantiFluo™ trypsin inhibitor assay kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate.
The fluorescein label is highly quenched. Upon digestion by trypsin present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.