The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.

• Alternative names: SPIKE_SARS2 Spike glycoprotein
• UniProt Number: P0DTC2
• Protein class: Single span transmembrane protein
• Organism: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
• Sequence, explained: Full-length sequence (aa 1 - 1273), furin cleavage site 'RRAR' mutated to 'GSAG'; C-terminal Rho1D4 tag fused with spacer 'GSSG' to protein sequence
• Affinity tags: C-terminal Rho1D4-tag
• Expression host: Hek293 Expi cells
• Buffer composition: 20 mM Hepes pH 7.5; 150 mM NaCl
• Stabilizing MSP nanodisc: MSP1D1-His, assembly with POPC lipids (Membrane scaffold protein based nanodisc; MSP1D1 = 253 kDa)
• Purified via: PureCube Rho1D4 Agarose
• Function: Host cell surface receptor binding; fusion of virus membrane with host endosome membrane

The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE this trimer needs to be kept intact. And Cube Biotech offers stabilized intact form of SPIKE.