Halobacterium salinarum S9 Native Bacteriorhodopsin

# de catalogue 77392-866

Fournisseur: CUBE BIOTECH

New Product

Numéro CAS:

Halobacterium salinarum S9 Native Bacteriorhodopsin

# de catalogue 77392-866

Fournisseur: CUBE BIOTECH

New Product

Numéro CAS:

Spécifications

Protein/peptide type:
Native
Size:
500 µg
Amino acid number:
262
Protein synonyms:
BR, HsBR, Bacteriopsin
Protein/peptide name:
Bacteriorhodopsin
Purity:
>95%, determined via SDS-PAGE
Molecular weight:
28256 Da
Sequence:
MLELLPTAVE GVSQAQITGR PEWIWLALGT ALMGLGTLYF LVKGMGVSDP DAKKFYAITT LVPAIAFTMY LSMLLGYGLT MVPFGGEQNP IYWARYADWL FTTPLLLLDL ALLVDADQGT ILALVGADGI MIGTGLVGAL TKVYSYRFVW WAISTAAMLY ILYVLFFGFT SKAESMRPEV ASTFKVLRNV TVVLWSAYPV VWLIGSEGAG IVPLNIETLL FMVLDVSAKV GFGLILLRSR AIFGEAEAPE PSAGDGAAAT SD
Cat. no.:
77392-866

Spécifications

A propos de cet article

Bacteriorhodopsin (BR) is a 7-transmembrane protein that acts as a light-driven proton pump in archaebacteria.

Alternative names: Bacteriorhodopsin, BR, HsBR, Bacteriopsin
UniProt Number: P02945
Protein class: 7-transmembrane protein
Organism: Halobacterium salinarum S9
Sequence, explained: Full-length, wildtype sequence with N-terminal signal sequence which is mostly absent in the preparation
Affinity tags: None
Expression host: Halobacterium salinarum S9
Buffer composition: Purple membrane (PM) patches in H₂O, 0.02% NaN₃
Purified via: Sequential centrifugation
Absorbance: Extinction coefficient at 560 nm: 63000 M⁻¹cm⁻¹
Activity: Binding of ligand all-trans retinal, covalently bound to a lysine residue
Evaluation of UV-VIS spectrum: absorbance ratio 280/560 nm = 2.3
Function: Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven proton pump

Due to BR's covalently bound colored ligand, BR is widely used as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide BR of high purity, prepared as purple membrane patches from Halobacterium salinarum.

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