Halobacterium salinarum S10 Recombinant Sensory Rhodopsin-1 (from E. coli)

# de catalogue 77392-868

Fournisseur: CUBE BIOTECH

New Product

Numéro CAS:

Halobacterium salinarum S10 Recombinant Sensory Rhodopsin-1 (from E. coli)

# de catalogue 77392-868

Fournisseur: CUBE BIOTECH

New Product

Numéro CAS:

Spécifications

Protein/peptide type:
Recombinant
Source:
E. coli
Size:
100 µg
Amino acid number:
234
Protein synonyms:
SR-1, HsSRI
Protein/peptide name:
Sensory Rhodopsin-1
Purity:
>98%
Molecular weight:
25423 Da
Sequence:
MDAVATAYLG GAVALIVGVA FVWLLYRSLD GSPHQSALAP LAIIPVFAGL SYVGMAYDIG TVIVNGNQIV GLRYIDWLVT TPILVGYVGY AAGASRRSII GVMVADALMI AVGAGAVVTD GTLKWALFGV SSIFHLSLFA YLYVIFPRVV PDVPEQIGLF NLLKNHIGLL WLAYPLVWLF GPAGIGEATA AGVALTYVFL DVLAKVPYVY FFYARRRVFM HSESHHHHHH HHHH
Cat. no.:
77392-868

Spécifications

A propos de cet article

Sensory rhodopsin-1 (SR-1) is a 7-transmembrane protein that acts as a phototaxis receptor in archaebacteria.

Alternative names: Sensory rhodopsin-1 (SR-1, HsSRI)
UniProt Number: P0DMH8
Protein class:7-transmembrane protein
Organism: Halobacterium salinarum S9
Sequence, explained: Wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10× his-tag
Affinity tags:His-tag (C-terminus)
Expression Host: E.coli
Buffer composition: 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Purified vial: PureCube Ni-NTA Agarose
Absorbance: Extinction coefficient at 560 nm: 63000 M⁻¹ cm⁻¹
Activity: Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280/ 590 nm = 1.7
Function: Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor

Due to Sensory rhodopsin-2's covalently bound colored ligand, SR-2 is ideal for use as a control membrane protein, for example, for crystallization assays and biochemical/biophysical experiments. We provide SR-1 of high purity, purified from E. coli as a his-tagged protein.

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