This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1E3D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

• Purity: >90% (Determined by SDS-PAGE)
• Number of amino acids: 277
• Molecular mass: 32600 Da
• Extinction coefficient (in water) ε280: 29400 M⁻¹cm⁻¹
• Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
• Sequence (Human origin): GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ
• Affinity tag: N-Terminal His-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences to stabilize membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1E3D1 (His-tagged) creates nanodiscs of ∼12 to 14 nm in diameter.

With this assembly kit both cellular as well as cell-free expressed proteins can be stabilized in MSP nanodiscs. Just use the corresponding protocol for each purpose (see below). The MSP protein contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.