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Spécifications
- Cat. no.:77393-512
- Contents:2×50 ml binding buffer; 2×50 ml wash buffer; 1×50 ml elution buffer; 1×10 ml Ni-INDIGO resin; 50×MINI batch spin tubes; 50×collection tubes; 1×500 mM DTT (add 1.016 ml ddH₂O); 1×500 mM EDTA (add 0.866 ml ddH₂O)
Spécifications
A propos de cet article
The HisCube His-tag protein purification MINI Kit is our way to supply your laboratory with a convenient method for high-quality, small-scale His-tag protein purifications. We put all of our experience in His-tag purifications into this Kit to provide a protocol that is easy to follow.
- Usage: Specific binding and purification of 6× His tagged proteins
- Specificity: Affinity to His-tagged proteins
- Binding capacity:>100 mg/ml
- Bead Ligand: INDIGO-Ni
- Bead size: 90 μm
- Filling quantity: Delivered as a 50% suspension
- pH stability: 2 - 14
- Chelator stability (e.g. EDTA): 20 mM
- Reducing agent stability (e.g. DTT): 20 mM
- Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Features - MagBead separator:
Usage: Separation of magnetic beads from the supernatant
Separation of magnetic beads from the supernatant: 1.5 and 2 ml microcentrifuge tubes (Eppis)
Design: Modular (2 microcentrifuges tubes/module): Can be put together onto a larger rack easily
Magnet strength: Above average. To ensure no magnetic beads get lost during the separation process
Area covered on work bench: 3 cm² per module
Cap holder: Included, so that microcentrifuge tubes do not fall close if not wanted
Color: White
The HisCube Ni-INDIGO MINI kit contains our in-house developed PureCube 100 Ni-INDIGO agarose resin and our PureCube 1-Step batch MINI batch spin tubes.
INDIGO's most significant advantage is its tolerance towards reducing and chelating agents. These can be used to great help in His-tag purifications, but traditional His-tag affinity beads do not tolerate them. EDTA is a chelator often used in eukaryotic cell buffers because it has protease-inhibiting functions. DTT can dissolve protein aggregates that could hinder the access of the purification beads to the His-tagged proteins. However, thanks to INDIGO, both substances can be used now. Therefore, they are part of the kit.
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