Membrane scaffold protein; rat origin.

• Usage: Specific binding and purification of Rho1D4-tagged proteins
• Specificity: Affinity to Rho1D4-tagged (TETSQVAPA) proteins
• Binding capacity: >3 mg/ml
• Bead ligand: anti-Rho1D4 antibody
• Bead size: 100 μm
• Matrix: 6% cross-linked agarose
• Recommended flow rates: 0.25 to 1 ml/min
• Max. flow rates: 6 ml/min
• Filling quantity: Delivered as a 50% suspension
• Short-term storage: In neutral buffer at 4 °C
• Long-term storage: In neutral buffer with 20% ethanol at 4 °C

Required equipment:
- Lysis buffer
- Wash buffer
- Elution buffer (incl. Rho1D4 peptide)
- Ice bath
- Refrigerated centrifuge for 50 ml tube (min 10000×g)
- 50 ml centrifuge tube
- Micropipettor and micropipetting tips
- Disposable gravity flow columns with capped bottom outlet, 2 ml
- pH meter
- End-over-end shaker
- SDS-PAGE buffers, reagents and equipment optional: Western Blot reagents and equipment

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1E3D1 (rat) creates nanodiscs of ∼12 to 14 nm in diameter. This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein. There are multiple phospholipids to choose from that can make up the body of the nanodisc. Which is best, depends on your protein.