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The PureCube Zn-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Zn-NTA resin. You can choose between two sizes, 1 and 5 ml bed volume.
- Usage: Specific binding and purification of 6× His-tagged proteins
- Binding of Zinc-binding proteins
- Specificity: Affinity to His-tagged proteins
- Affinity to Zinc-finger proteins
- Binding capacity: >50 mg/ml
- Bead Ligand: Zn-NTA
- Bead size: 40 μm
- Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
- Filling quantity: Delivered as a 50% suspension
- Required equipment and materials:
- FPLC hardware like e.g. ÄKTA
- Binding Buffer
- Elution Buffer
Please have a look at the given features table to ensure that the Cartridge/Column is compatible with your FPLC hardware. Common systems like ÄKTA TM are compatible without problem. If this is not the case we recommend buying the sole loose PureCube Zn-NTA resin and packing the columns yourself. Alternatively, we also offer our PureCube Zn-NTA products as magnetic beads, if you prefer this IMAC method.
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