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The PureCube Co-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Co-NTA resin.
- Usage: Specific binding and purification of 6× His tagged proteins
- Specificity: Affinity to His tagged proteins
- Binding capacity: >30 mg/ml
- Bead Ligand: Co-NTA
- Bead size: 40 μm
- Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
- Filling quantity: Delivered as a 50% suspension
- Required equipment and materials:
- FPLC hardware like e.g. ÄKTA
- Binding Buffer
- Elution Buffer
You can choose between two sizes:1 ml bed volume and 5 ml bed volume. The PureCube cartridge/column used here is compatible with almost all commonly used FPLC systems like ÄKTA. If not, we recommend buying the sole loose PureCube Co-NTA resin and packing the columns yourself. Alternatively, we offer our PureCube Co-NTA products as magnetic beads if you prefer this IMAC method.
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