The OsteoImage® mineralization assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralization. The OsteoImage® assay can quantitate in vitro mineralization by osteogenic stem cells, primary osteoblasts, and osteoblast-like cell lines.

• Delivers qualitative visual fluorescent microscopy or quantitative fluorescent plate reader results
• Can be used with primary osteoblasts, osteoblast stem cells, and osteoblast cell lines
• Measures hydroxyapatite, similar to real bone
• Completed in less than 90 minutes, without tedious extractions
• Sensitive enough to detect time dependent increases in mineralization in differentiating cells
• Scalable for use in 6-well to 96-well plates

It is based on specific binding of the fluorescent OsteoImage® Staining Reagent to the hydroxyapatite portion of bone-like nodules deposited by cells.

Unlike typical histochemical methods such as von Kossa and Alizarin red, neither of which is hydroxyapatite specific, the OsteoImage® assay eliminates multiple steps or tedious extraction steps.

Increase the speed, sensitivity, and ease of measuring mineralization in your cell cultures with the OsteoImage® mineralization assay.