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Spécifications
- Assay duration:One step
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:7.18 ng/L
- Target protein:CTCF
- Description:Human CTCF ELISA kit
- Size:96 tests
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:100 - 1600 ng/L
- Storage temperature:4 °C
- Sample volume:40 μl
- Regulatory status:RUO
- Cat. no.:77210-114
Spécifications
A propos de cet article
Human CTCF ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CTCF in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutess, with a single wash step
- Detection Range: 100 - 1600 ng/L
- Sensitivity: 7.18 ng/L
- Sample Type: Serum, plasma or other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human CTCF ELISA kit (A312243) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CTCF in serum, plasma or other biological fluids. An antibody specific for CTCF has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CTCF present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-CTCF antibody, which also binds the CTCF present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-CTCF antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-CTCF antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of CTCF captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of CTCF in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.