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Spécifications
- Assay duration:One step
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:5.18 ng/L
- Target protein:BMP15
- Description:Human BMP15 ELISA kit
- Size:96 tests
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:75 - 1200 ng/L
- Storage temperature:4 °C
- Sample volume:40 μl
- Regulatory status:RUO
- Cat. no.:77211-846
Spécifications
A propos de cet article
Human BMP15 ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human BMP15 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human BMP15 ELISA kit (A312864) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human BMP15 in serum, plasma or other biological fluids. An antibody specific for BMP15 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the BMP15 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-BMP15 antibody, which also binds the BMP15 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-BMP15 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-BMP15 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of BMP15 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of BMP15 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.