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Spécifications
- Assay duration:One step
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:0.023 ng/ml
- Target protein:ADCY10/SAC
- Description:Human ADCY10/SAC ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma or other biological fluids
- Detection method:Colorimetric
- Time to Results:1 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:1 - 16 ng/ml
- Storage temperature:4 °C
- Sample volume:40 μl
- Regulatory status:RUO
Spécifications
A propos de cet article
Human ADCY10/SAC ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human ADCY10/SAC in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human ADCY10/SAC ELISA kit (A312106) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human ADCY10/SAC in serum, plasma or other biological fluids. An antibody specific for ADCY10/SAC has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ADCY10/SAC present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-ADCY10/SAC antibody, which also binds the ADCY10/SAC present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-ADCY10/SAC antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-ADCY10/SAC antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of ADCY10/SAC captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of ADCY10/SAC in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.