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Spécifications
- Description:miniPCR® plant genetics lab
- No. of Lab Groups:8
- Cat. no.:470351-998
Spécifications
A propos de cet article
Investigate the genotypic basis of a phenotype using Wisconsin Fast Plants®.
- Techniques: Micropipetting, DNA extraction, PCR, gel electrophoresis
- Topics: Genetic inheritance, genotype to phenotype, plant biotechnology
- Time required: Can be completed in two 45-minute class periods or one 120-minute class period
- Level: General high school through college
Investigate the genotypic basis of an observable phenotype using Rapid Cycling Brassica rapa (RCBr), also known by the trademark name Wisconsin Fast Plants®. Wild-type plants grow with a distinctive purple stem due to the pigment anthocyanin. In some RCBr plants, a mutation disrupts the anthocyanin production pathway and leads to green stems. The gene responsible for the purple vs. green color is named Anthocyaninless because the mutant form leads to decreased production of the pigment anthocyanin. Test the Anthocyaninless gene from different plants to link genotype to phenotype.
Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H₂O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes
- PCR tubes
Required equipment:
- Micropipettes and tips
- Thermal cycler
- Gel electrophoresis and visualization system