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- Description:miniPCR® forensics lab D1S80 VNTR
- No. of Lab Groups:8
- Cat. no.:470351-996
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A propos de cet article
A crime has occurred in your classroom!
- Techniques: Micropipetting, DNA extraction, PCR, gel electrophoresis
- Topics: forensics, genetic polymorphism, biotechnology
- Time required: Can be completed in two 45-minute class periods or one 90-minute class period
- Level: General high school through college
Students will use PCR to amplify their own DNA and compare it to a sample obtained from a hypothetical crime scene to try to rule themselves out as a suspect. Variable number tandem repeats (VNTRs) are regions in a genome that contain short stretches of DNA repeated a number of times. The number of repeats in a particular VNTR can vary from individual to individual. In this way, genetic variation in VNTRs can be used as markers for personal identification. This hands-on activity lets students explore molecular biology in personal identification and forensics, as well as inheritance, human genetics, DNA polymorphisms, and genetic diversity.
Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H₂O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes
- PCR tubes
Required equipment:
- Micropipettes and tips
- Thermal cycler
- Gel electrophoresis and visualization system
Storage notes:
- Reagents require freezer storage
- Reagents are stable for 12 months when stored in the freezer