Missy Baker is missing!

• Techniques: Micropipetting, PCR, gel electrophoresis
• Topics: Forensics, genes and disease, genotype to phenotype, cystic fibrosis, biotechnology
• Time required: Can be completed in two 45-minute class periods or one 90-minute class period
• Level: General high school through advanced high school

Missy Baker is missing and police have two suspects. Knowing that Missy Baker has cystic fibrosis, students will test DNA evidence from both suspects’ cars to see if it matches a person with a CFTR deletion. This lab provides an excellent overview of genetic technologies and connects their uses, from identification to genetic diagnosis, in an engaging and accessible format. With reliable results and a straightforward procedure, Missy Baker Missing is an excellent lab to introduce students to PCR and gel electrophoresis lab techniques.

Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H₂O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes
- PCR tubes

Required equipment:
- Micropipettes and tips
- Thermal cycler
- Gel electrophoresis and fluorescence visualization system

Storage notes:
- Reagents require freezer storage
- Reagents are stable for 12 months when stored in the freezer