Molly the Labradoodle has surprised you with a litter of puppies!

• Techniques: Micropipetting, gel electrophoresis
• Topics: Mendelian inheritance, genotype to phenotype, biotechnology
• Time required: Can be completed in one 45-minute class period
• Level: General high school through advanced high school

Students will use gel electrophoresis to examine the link between genotype and phenotype in Molly’s puppies. By tracking the inheritance of a single trait, students can determine the puppies’ father. This lab allows you to cover Mendelian genetics and the relationship between genotype and phenotype using an engaging and adorable case study. An extension activity allows students to practice tracking the inheritance of multiple genes that affect dog coats.

Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H₂O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes

Required equipment:
- Micropipettes and tips
- Gel electrophoresis and fluorescence visualization system

Storage notes:
- Reagents require freezer storage
- Reagents are stable for 12 months when stored in the freezer