To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Spécifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Reactivity:Human
- Sensitivity:0.188 ng/ml
- Target protein:BCKDHA
- Description:Human BCKDHA ELISA kit
- Format:Pre-coated
- Size:96 tests
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Human BCKDHA ELISA Kit exhibits high specificity and excellent specificity for the detection of human BCKDHA. No significant cross-reactivity or interference between BCKDHA and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Type:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0.313 - 20 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Regulatory status:RUO
Spécifications
A propos de cet article
Human BCKDHA ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human BCKDHA in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human BCKDHA ELISA kit (A246910) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human BCKDHA in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for BCKDHA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the BCKDHA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-BCKDHA antibody, which binds the captured BCKDHA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of BCKDHA captured in each well. The concentration of BCKDHA can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.