A propos de cet article
ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.
- Highly sensitive, detect from a single cell to tens of thousands of cells
- Excellent linearity over 6 orders of magnitude
- Homogeneous, no-wash assay
- Detect ATP amount or quantify number of live cells
ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay procedure involves simply adding the ATP-Glo™ detection cocktail directly to cells cultured in complete culture medium. It is not necessary to remove medium or wash cells before adding the reagent.
Kit Components: D-Luciferin, Firefly luciferase (recombinant, produced in E. coli), ATP-Glo™ assay buffer and 2 mm ATP standard.
This ATP detection kit takes advantage of firefly luciferase’s use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available. Because ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP present.
The ATP-Glo™ kit can be used to detect as little as a single cell or 0.01 picomole of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.