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A competitive assay for the quantification of tetranor-PGDM in urine.
- Detect tetranor-PGDM, a major metabolite of PGD2 in urine
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure tetranor-PGDM levels down to 40 pg/ml
- Read: Colorimetric at 405 to 420 nm
tetranor-PGDM is a major metabolite of prostaglandin D2 (PGD2) found in human and mouse urine. In human urine, tetranor-PGDM is significantly more abundant than the PGD2 metabolites 11β-PGF2α and 2,3-dinor-11β-PGF₂α and is the only endogenous PGD2 metabolite detectable in mouse urine by LC-MS.
Normal levels of tetranor-PGDM in human and mouse urine are 1.5 ng/mg creatinine and 8.1 ng/mg creatinine respectively. tetranor-PGDM ELISA Kit is a competitive assay that can be used for quantification of tetranor-PGDM in urine. This assay converts tetranor-PGDM to a stable derivative, tetranor-PGJM, that can be easily quantified.
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