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Spécifications
- Storage temperature:−80 °C
- Cat. no.:75817-034
- Supplier No.:600590-96
- No. of Tests:96 Wells
Spécifications
A propos de cet article
A 96-well assay for measurement of Nrf2.
- A sensitive, non-radioactive method of detecting Nrf2 from whole cell lysates
- 96-well plate format replaces EMSAs
- Capture the transcription factor using a specific dsDNA sequence bound to the plate
- Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
Nuclear factor E2-related factor 2 (Nrf2) is a transcription factor that plays a key role in maintaining redox homeostasis via its interaction with a cysteine-rich protein Kelch-like ECH-associated protein 1 (Keap1). In resting cells, Nrf2 and Keap1 form a tight complex, which is targeted for degradation by proteasomes.
Under oxidative stress, Nrf2 is released from the Nrf2/Keap1 complex and translocate to the nucleus where it is able to induce the expression of a battery of genes encoding diverse cytoprotective proteins, including antioxidative enzymes, anti-inflammatory mediators, and proteasomes. Nrf2 Transcription Factor Assay is a non-radioactive, colorimetric method for detecting specific transcription factor DNA binding activity in nuclear extracts.
A specific double stranded DNA (dsDNA) sequence containing the Nrf2 response element is immobilized onto the wells of a 96-well plate. Nrf2 contained in nuclear extract samples will bind specifically to the Nrf2 response element and can be detected by addition of a specific Nrf2 antibody and a secondary antibody conjugated to HRP.