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This kit is used for the measurement of 13,14-dihydro-15-keto prostaglandin F2α in plasma.
- Detect 13,14-dihydro-15-keto PGF2α, a stable metabolite of PGF2α
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure 13,14-dihydro-15-keto PGF2α levels down to 16.3 pg/ml
- Reads colorimetric at 405 to 420 nm
PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2α in vivo, by the enzymes 15-hydroxy PG dehydrogenase and D13-reductase. Measurement of 13,14-dihydro-15-keto PGF2α in plasma can be used as a marker of the in vivo production of PGF2α.
13,14-dihydro-15-keto PGF2α ELISA is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF2α within the range of 2.3 to 5000 pg/ml. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF2α in plasma, a common matrix for measurement of this PGF2α metabolite.
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