To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Spécifications
- Description:Chemiluminescent Western Blot Kit: DYKDDDDK (FLAG®) Chemiluminescent Western Blotting Kit
- Size:1 Kit
- Shipping temperature:Wet Ice
- Cat. no.:RLKCA383
- Supplier No.:KCA383
Spécifications
A propos de cet article
DYKDDDDK (FLAG®) Chemiluminescent Western Blotting Kit
- This Chemiluminescent Western Blot Kit allows for the detection of FLAG® -tagged recombinant protein present in cell lysates provided by the user.
- contains all of the necessary reagents
- rapid proven protocol
- extremely high signal detection of our luminol chemiluminescent substrate for the detection of FLAG® -tagged recombinant protein
The FLAG® epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG® -tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG®) antibodies. The anti-DYKDDDDK (FLAG®) antibody included in this Western blot kit detects FLAG® -tagged recombinant proteins fused with FLAG® tag either at the amino-terminus or carboxy-terminus of the protein.
Use Rockland Immuno-chemicals’ Anti-DYKDDDDK (FLAG®) Chemiluminescent Kit for Western Blotting for detection of FLAG® -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG®). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.