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Spécifications
- Size:192 Wells
- Cat. no.:75817-192
- Contents:Buffer, Heme, COX-1 (ovine), COX-2 (human), Arachidonic Acid (substrate), Potassium Hydroxide, Substrate, Plate, and Cover Sheet
- Supplier No.:701050-192
Spécifications
A propos de cet article
A colorimetric assay for the screening of isozyme-specific inhibitors.
- Screen for inhibitors of ovine COX-1 or human COX-2
- Measure the peroxidase component of the COX
- Assay 41 samples for COX-1 and 41 samples for COX-2 in duplicate
- Plate-based colorimetric measurement (590 nm)
Cayman's COX Colorimetric Inhibitor Screening Assay measures the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. Inhibition of COX activity, measured by TMPD oxidation, by a variety of selective and nonselective inhibitors, shows changed potencies similar to those observed with other in vitro methods. The COX Colorimetric Inhibitor Screening assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. Cayman's COX Colorimetric Assay is a time saving tool for screening vast numbers of inhibitors.