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Spécifications
- Brand name:Cayman
- Cat. no.:75816-766
- Contents:Kit contains: Buffers, Standard, Detector, Horseradish Peroxidase, Reagent, and Plate
- Supplier No.:10010895-96
Spécifications
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For analysis of xanthine oxidase in plasma, serum, and tissue homogenates
- Measure XO activity in plasma, serum, and tissue homogenates
- Assay 42 samples in duplicate
- Measure XO activity down to 10 µU/ml
- Plate-based fluorometric measurement (ex 520-550 nm, em 585-595 nm)
XO catalyzes the hydroxylation of hypoxanthine to xanthine and then further catalyzes the oxidation of xanthine to uric acid. When oxidizing NADH, XO generates superoxide, a powerful ROS. XO is found predominantly in the liver and intestine. However, levels of circulating XO can increase dramatically with disease. Cayman’s XO Assay provides a simple and accurate method for quantifying xanthine oxidase activity. The assay is based on a multistep enzymatic reaction whose end product resorufin, a highly fluorescent compound, can be easily analyzed using an excitation wavelength between 520-550 nm and an emission wavelength between 585-595 nm.