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Spécifications
- Brand name:Cayman
- Cat. no.:75817-222
- Contents:Kit contains: Buffer, Standard, Reagents, and Plate
- Range:5 - 25 µM (nitrate and/or nitrite)
- Supplier No.:760871-96
Spécifications
A propos de cet article
A complete assay kit for measurement of NO2/NO3
- Designed to measure NOS activity in vitro
- Can also be used to measure nitrate and nitrite in urine, plasma, serum, and tissue homogenates
- Assay 41 samples in duplicate
- Plate-based colorimetric measurement (530-550 nm)
NADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a NOS-catalyzed reaction, thereby making the assay particularly well suited to measurements of NOS activity in vitro. High throughput screening applications using recombinant NOS preparations can be quickly and accurately assayed with the LDH method kit.