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A sensitive assay for the detection of 11β-PGF2α
- Detect 11β-PGF2α, the primary metabolite of PGD2, in plasma or urine
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure 11β-PGF2α levels down to 5 pg/ml
- Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
- Midpoint: 50% B/B0: 25-45 pg/ml
11β-PGF2α is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excretion of 11β-PGF2α is about 11 ng/mmol creatinine (~400 ng/24 hr), which is increased nearly 3-fold upon allergen-induced bronchoconstriction in asthmatics. Cayman’s 11β-PGF2α Assay is a competitive assay that provides accurate measurements of 11β-PGF2α within the range of 1.6-1,000 pg/ml.
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