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Spécifications
- Cat. no.:75817-086
- Includes:Kit contains: A-23187, Substrate, Reagents, and Plate
- Supplier No.:601010-1
Spécifications
A propos de cet article
For the measurement of NET-derived neutrophil elastase.
- Induce and detect NET formation in vitro
- Elastase-based readout
- Non-dsDNA readout eliminates false positives from DNA
- Adaptable to multiple species
Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). Unbound neutrophil elastase is washed away following NET generation. Following digest of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by elastase to yield a 4-nitroaniline product that adsorbs light at 405 nm. Enough reagents are provided to test 24 sample conditions for NET production, with analysis in duplicate.