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Spécifications
- Cat. no.:75817-252
- Includes:Kit contains: Positive Control, Wash Solution, and Reagents
- Supplier No.:10012643-1
Spécifications
A propos de cet article
A colorimetric screening assay for hepatoxicity assessment.
- Detect excessive lipid accumulation in cells
- Assess by microscopy or plate reader
- An inducer of lipid accumulation is included as a positive control
Steatosis, also known as fatty liver, is a pathological process characterized by abnormal accumulation of lipid within cells. While simple steatosis may not be associated with significant impairment of liver function, excessive fat accumulation can lead to cirrhosis and even liver failure. The molecular mechanisms involved in non-alcoholic steatosis are poorly understood and little information is available on the pathway(s) responsible for progressive hepatocellular damage following lipid accumulation. Determining hepatotoxicity is an essential component of the drug discovery process and includes steatosis as one of the key parameters evaluated. Cayman’s Steatosis Colorimetric Assay Kit provides a convenient tool for evaluating steatosis risk of drug candidates. In this assay, Oil Red O is used to stain neutral lipids in hepatocytes. Lipid accumulation is then quantified using a plate reader after the dye is extracted from the lipid droplets. Chloroquine is included in the kit as a positive control.