Order Entry
ContactUsLinkComponent
NF-κB (p65) Transcription Factor Assay Kit, Cayman Chemical Company
NF-κB (p65) Transcription Factor Assay Kit, Cayman Chemical Company
# de catalogue 75816-704
Numéro CAS:  
NF-KB (p65) Transcription Factor Assay Kit
NF-κB (p65) Transcription Factor Assay Kit, Cayman Chemical Company
# de catalogue 75816-704
Numéro CAS:  
Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

  • • State issued document with your organization's Federal Tax ID Number
  • • State issued document with your organization's Resale Tax ID Number
  • • City or County issued Business License
  • • State Department of Health Services License
  • • Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.

Spécifications

  • No. of wells:
    96
  • Storage temperature:
    −80 °C
  • Cat. no.:
    75816-704
  • Supplier No.:
    10007889-96

Spécifications

A propos de cet article

A 96-well assay for measurement of NF-κB (p65).

  • A sensitive, non-radioactive method of detecting NF-κB (p65) from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format

Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p65) is detected by addition of specific primary antibody directed against NF-κB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s NF-κB (p65) Transcription Factor Assay detects human NF-κB (p65). It will not cross-react with NF-κB (p50).

Ordering information: Nuclear Extraction Kit is available separately to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates.
Delivery: Kit contains binding assay buffer, Reagent A, positive control, antibody binding buffer, primary antibody, buffer concentrate, polysorbabte 20, competitor dsDNA, HRP conjugate, strip plate, cover sheet, developing and stop solution.
Customers Who Bought This Also Bought