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Spécifications
- Description:Phosphatase Assay Kit
- Size:500 assays
- Method:OD405 nm
- Sample:Protein phosphates
- Processing time:30 minutes
- Cat. no.:75878-352
- Supplier No.:POPN-500
Spécifications
A propos de cet article
For quantitative determination of phosphatase activity.
- The detection limit is generally 3 ng phosphatase or below
- No wash or reagent transfer steps are involved
- All reagents are compatible with high-throughput liquid handling instruments
p-Nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The reaction yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer. This homogeneous "mix-and-measure" assay involves simply adding a single reagent to the phosphatase and measuring the product formation on an absorbance reader. The assay can be conveniently performed in cuvettes, tubes or multi-well plates at either room temperature or 37°C.