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Spécifications
- Description:Sialic acid Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Biological
- Processing time:Two hours
- Cat. no.:75878-170
- Supplier No.:ESLA-100
Spécifications
A propos de cet article
For quantitative determination of free and total sialic acid (NANA) concentration.
- Linear detection range 20 - 1000 μM sialic acid for colorimetric assays and 2 - 100 μM for fluorimetric assays
- Can detect both free and total sialic acid
Sialic acid is a general name for nine carbon acidic sugars with N- or O-substituted derivatives
BioAssay Systems’ sialic acid assay uses a single Working Reagent that combines NANA aldolase, pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to sialic acid concentration in the sample.
The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The Sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple, direct and automation-ready. Procedures for measuring sialic acid concentrations find wide applications in research and drug discovery.