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Spécifications
- Description:Phospholipase D Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Biological
- Processing time:30 minutes
- Cat. no.:75878-162
- Supplier No.:EPPD-100
Spécifications
A propos de cet article
For quantitative determination of phospholipase D activity.
- Detection range colorimetric assay 0.06 - 10 U/L, fluorimetric assay 0.04 - 1 U/L
- Can be readily adapted to high-throughput assays for drug screening
Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup
BioAssay Systems’ method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
Abnormalities in PLD expression have been associated with human cancers.