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Spécifications
- Description:Phospholipid Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Biological
- Processing time:20 minutes
- Cat. no.:75878-160
- Supplier No.:EPLP-100
Spécifications
A propos de cet article
For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.
- Linear detection range colorimetric assay 3 - 200 μM, fluorimetric assay 0.6 - 20 μM phospholipid
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction
BioAssay Systems’ method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
Most phospholipids contain one diglyceride, a phosphate group, and one choline.