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Spécifications
- Description:Malate Dehydrogenase Assay Kit
- Size:100 assays
- Method:OD565 nm
- Sample:Biological Samples (e.g. plasma, serum, erythrocytes, tissue and culture media).
- Processing time:30 minutes
- Cat. no.:75878-150
- Supplier No.:EMDH-100
Spécifications
A propos de cet article
For quantitative determination of L-malate dehydrogenase enzyme activity.
- Linear detection range 0.5 to 65 U/L.
- Homogeneous "mix-incubate-measure" type assay
- Can be readily automated to test thousands of samples per day
Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD
BioAssay Systems’ non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer’s disease.