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Spécifications
- Description:Invertase/Sucrase Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm
- Sample:Biological, environment (soil) etc.
- Processing time:40 minutes
- Cat. no.:75878-142
- Supplier No.:EIVT-100
Spécifications
A propos de cet article
For quantitative determination of invertase/sucrase activity.
- Safe
- Non-radioactive assay
- "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved
- Can be readily automated to test thousands of samples per day
Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose
BioAssay Systems’ Invertase Assay Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (λex/em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.
Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural and food (confectionery) industry.