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Spécifications
- Description:Isocitrate Assay Kit
- Size:100 assays
- Method:FL530/585 nm
- Sample:Food, beverage, biological Samples (e.g. cell lysate, tissue homogenate, serum, etc.).
- Processing time:10 minutes
- Cat. no.:75878-086
- Supplier No.:EFIC-100
Spécifications
A propos de cet article
For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism.
- Linear detection range 0.6 to 500 μM for 10 min reaction
- Homogeneous "mix-incubate-measure" type high-throughput assay
Isocitrate (Isocitric acid) is a substrate in the citric acid (TCA) cycle
BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.
Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.