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Spécifications
- Description:Cell Viability Assay Kit
- Size:100 assays
- Method:Luminescence
- Sample:Cells
- Processing time:Two minutes
- Cat. no.:75878-080
- Supplier No.:ECTX-100
Spécifications
A propos de cet article
For rapid, bioluminescent determination of cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents.
- Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay)
- As low as 50 cells can be quantified
- "Mix-incubate-measure" high-throughput assay
Adenosine 5’-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell
BioAssay Systems’ EnzyLight™ Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells. This non-radioactive, homogeneous cell-based assay can be conveniently performed in microplates. The reagent is compatible with all culture media and liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.