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Spécifications
- Description:Coenzyme A Assay Kit
- Size:100 assays
- Method:OD570 nm, or FL530/585 nm.
- Sample:Biological
- Processing time:60 minutes
- Cat. no.:75878-074
- Supplier No.:ECOA-100
Spécifications
A propos de cet article
For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism.
- Linear detection range colorimetric assay 5 - 1000 μM, fluorimetric assay 3 - 100 μM CoA
- Convenient room temperature "mix-and-read" assay
- Can be readily automated to process thousands of samples per day
Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others
BioAssay Systems’ method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.
One of CoA’s most crucial roles is the carrying and transferring of acyl groups.